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Thursday, December 3, 2020 | History

2 edition of Rapid color method for differentiation of fat in gross specimens found in the catalog.

Rapid color method for differentiation of fat in gross specimens

J. Kaufmann

Rapid color method for differentiation of fat in gross specimens

  • 354 Want to read
  • 34 Currently reading

Published by s.n. in [S.l .
Written in English

    Subjects:
  • Anatomical specimens -- Collection and preservation.

  • Edition Notes

    Other titlesBulletin of the International Association of Medical Museums.
    Statementby J. Kaufmann and E. L. Judah.
    ContributionsJudah, Ernest Lionel.
    The Physical Object
    Pagination1 sheet ;
    ID Numbers
    Open LibraryOL18610772M

    The TF (Thomson – Friedenreich) blood group antigen behaves as an onco-foetal carcinoma-associated antigen, showing increased expression in malignancies and its detection and quantification can be used in serologic diagnosis mainly in adenocarcinomas. This study was undertaken to analyze the sera and tissue level detectable mucin-type glycoprotein (TF-antigen) by Peanut agglutinin (PNA) and. Methyl red produces a color change from red to yellow in the pH range 4 to 6, and bromthymol blue turns from yellow to blue in the range of 6 to 9. Therefore, in the pH range 5 to 9 measured by the reagent strips, one sees colors progressing from orange at pH 5 through yellow and green to a final deep blue.


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Rapid color method for differentiation of fat in gross specimens by J. Kaufmann Download PDF EPUB FB2

Thus far, only the Foss-Let method for fat has been accepted [A.O.A.C. as a rapid alternative fat method. Rapid determination of results may allow adjustments of processing directly on the production line. On-line scanners connected to computer control can facilitate rapid adjustment of raw material streams into blendsCited by: 6.

The color photography of gross specimens. Vetter JP. A method of color preservation and photography of gross specimens is presented in this article. The natural color and physical consistency of the preserved specimens assure "true to life" color photographs.

The author explains how his institution's system of combining the Autopsy Review Cited by: 1. CLG-FAT Page 4 of 8. Title: Determination of Fat Revision: 03 Replaces: CLG -FAT Effective: 08/10/ iii. Roll edges of dish. Dry the folded dish (on a metal mesh tray) in a mechanical convection oven for 6 hours ± 10 minutes at °C or for 1½ hours ± 10 minutes at ± 1°C.

After cooling, insert into extraction thimble. This chapter provides an approach to the processing of gynecologic and obstetric tissue specimens. The techniques of gross examination and the method of reporting the pathologic findings are guided by the clinical principles on which patient management is based.

Several textbooks are now devoted entirely to this topic. 1–3. The reference used for compiling the methods in Section I is: Murray, P.R., Baron, E. J., Jorgensen, J.J., Pfaller, M.A., and Yolken, R.H. Manual of Clinical A definite color change that is not quite yellow may be interpreted as a weak positive reaction.

This test can be used for differentiated different bacteria. Specimen. With the various staining methods we can visualise the different parts of the cells. When observing a tissue sample under the light microscope, it is often difficult to distinguish between different cells and tissue, as they are almost colorless.

Therefore staining is used to create differential coloration, allowing clearer observation and analysis of cells. color. It is a pH dependent reaction and occurs in an alkaline solution. Troubleshooting.

Reddish color of a stained section is due to inadequate bluing. Bluing reagents should have a pH of approximately 8. It is not possible to over-blue a section. The bluing reagent can. Grossing • Grossing of specimen is important stage in surgical pathology.

• It involves: – Accurate naked eye description of intact specimen – Correct method of sectioning – Gross examination of cut surface – Selection of proper tissue blocks for microscopy – Instructions for.

Degreasing and bleaching after removal of soft tissue by any above method bone is immersed in chloroform for hrs remove fat specimens are dried in a incubator bleached in Hydrogen peroxide Mounting Macerated bones are mounted dry.

Mounted on a central plate or on Perspex box The specimens fixed with nylon wire They are the routine manual, rapid manual and the microwave methods. This study aimed to proceed a simple new manual method in a trial to take the advantages of rapid manual and microwave methods.

In the case of hematoxylin, hydrochloric acid (for rapid differentiation) and acetic acid (for slower, more controlled differentiation) are most commonly used. While hydrochloric acid (HCl) has historically been the standard, milder acids are being used to provide gentler dye removal.

Make a longitudinal cut with a long knife along the long axis of the kidney to bivalve the specimen (i.e. open like a book) either from medial to lateral or vice versa Serially section the entire specimen, including perinephric fat, at 1 cm intervals either along the same plane as the first cut creating "pages in a book" (anterior to posterior.

Variation in color is due to the selective affinity of tissue components to hematoxylin. With the regressive method, overstaining the tissue section with a neutral hematoxylin solution is the initial step. An acid alcohol is then used to remove excess stain, followed by an alkaline solution to achieve a neutralized tissue section.

Staining methods / Staining of nerve tissue H&E staining. This is a standard staining method used in pathology. Typically, the cytoplasm of cells is eosinophilic (acidophilic) and is stained red, whereas the nuclei and nucleoli are “hematoxylinophilic” (basophilic) and are stained blue.

1. Introduction. Solid renal masses are rather common neoplasms occurring in adults that can be classified into benign and malignant histotypes [, ].Among benign histotypes, angiomyolipoma (AML) is the most common type, and oncocytoma is relatively uncommon that accounts for 3%–7% of solid renal masses, while leiomyoma is very rare [1,2].Renal cell carcinoma (RCC) is the most.

Pediatric color-coded Vacutainer® tubes are provided to facilitate special handling. Special small conical tubes with screw caps are provided to prevent evaporation of small volume samples. These tubes will hold up to mL of specimen. Standard specimen transfer tubes should be used for larger volume samples.

For urine specimens, use urine. Color is usually pale yellow/ amber and darkens when it becomes concentrated, but excessive fluid intake and some foods medications, stress, and exercise, may affect color. Urochrome is the pigment that gives urine its characteristic yellow color.

A variety of medications and other agents may cause the urine to change color. Specimens that are to be processed will be placed in suitable labelled cassettes (small perforated baskets) to segregate them from other specimens. The duration of the processing schedule used to process the specimens will depend on the type and dimensions of the largest and smallest specimens, the particular processor employed, the solvents.

FT-IR spectroscopy proved to be the most direct and accurate method of monitoring gross changes in the frying oil over time [24, 42]. A quantitative FTIR method was used for monitoring the oxidative state of frying oils, based on the determination of anisidine value (AV), a measure of aldehydes that are major secondary oxidation products in.

Gross Morphology and Angiogenic Effect of Engrafted Whole Fat and ADSC + Peptide Hydrogel Our results were consistent with well-documented effects of fat grafting's impact on surrounding, local tissue after fat engraftment.

8 – 16 Increased angiogenesis, or vessel growth from existing vasculature, was noted on the dermal side of experimental. The Gram stain is an empirical method for differentiating bacterial species into two large groups based on the chemical and physical properties of their cell walls.

Gram-positive bacteria retain the primary stain while gram-negative bacteria take the color of the counterstain. A Gram stain can also serve to assess the quality of a clinical. This method should be used for all geo-technical and general classification purposes.

Method D Determine the mass of acovered high-silica or porcelain dish. Place a part of or all of the oven-dried test specimen from amoisture determination inthedish and determine the mass ofthedish and specimen. Staining is a technique used to enhance contrast in samples, generally at the microscopic level.

Stains and dyes are frequently used in histology (the study of tissue under the microscope) and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of disease at a microscopic level.

Stains may be used to define biological tissues. A small portion of stool(fecal) specimen is applied to the paper. A developer solution containing hydrogen peroxide (H2O2) is added to the paper.

If the blood is present in the specimen, the iron (Fe) in the hemoglobin catalyses the reaction between guaiac in the paper and the H2O2. The completed reaction forms a blue color. Sensitive assays for rapid quantitative analysis of histologic sections, resected tissue specimens, or in situ tissue are highly desired for early disease diagnosis.

Stained histopathology is the gold standard but remains a subjective practice on processed tissue taking from hours to days. We describe a microscopy technique that obtains a sensitive and accurate color-coded image from intrinsic.

RAPID METHOD FOR THE DETERMINATION OF FAT IN FECES* BY J. VAN DE KAMER, H. TEN BOKKEL HUININK, AND H. WEYERS (From the Central Znstitute for Nutrition Research, Utrecht, The Netherlands) (Received for publication, Aug ) Study of fat absorption in a patient requires the determination of the fat.

Cell proliferation is responsible for the exponential increase in the cell number, resulting in rapid tissue growth. The process is balanced by cell division and cell differentiation or cell death, which maintains an Read more Cell proliferation- Definition, assay, differentiation, diseases.

Bone specimens should be sawn into thin slices using fine tooth saws prior to decalcification. The method is usually carried out between the stages of fixation and processing and is essential for good section preparation and is used for bone and other tissues that may contain calcified areas.

maximum diameter. The color, when re- corded by the clinician, was most often tan, pink to red, or skin colored.

On section of the gross specimen, the color most frequently re- ported was yellow, and less often gray. In 23 of 46 tumors the consistency was described as firm or. Hepatic Steatosis. Hepatic steatosis can be either diffuse or focal.

Focal steatosis is often easily recognized on the basis of the typical periligamentous or periportal location, the distribution of the lesions, and the presence of nondistorted, traversing blood vessels (, Figs 1, 2,).However, patchy focal fat deposition or sparing may be mistaken for an infiltrative neoplasm (, 5) (, Fig 3).

A pathology report is a medical document that gives information about a diagnosis, such as test for the disease, a sample of your suspicious tissue is sent to a lab.

FATF: Total fecal lipids include glycerides, phospholipids, glycolipids, soaps, sterols, cholesteryl esters, and sphingolipids.

Excess fecal fat in feces, (steatorrhea) is indicative of malabsorption disorders, such as pancreatic insufficiency or Whipple disease. Therefore, measurement of the fecal fats can be useful in establishing a diagnosis of such pancreatic diseases as cystic fibrosis.

exposure of the fat to the extraction solvent. For example, a cheese sample must be treated in several steps before solvent extraction.

The entire manual extraction process usually requires 2 to 3 h and more than mL of solvent per sample (AOAC Official Method ).2 Thus, the standard fat extraction methods, such as Roese-Gottlieb.

Rapid Urease Test (RUT) The rapid urease test (RUT) is a popular diagnostic test for diagnosis of Helicobacter pylori. It is a rapid, cheap and simple test that detects the presence of urease in or on the gastric mucosa. It is also known as the CLO test (Campylobacter-like organism test).

The LyssaChip developed in the current study represents a rapid, high-throughput, and economical method for the detection and differentiation of the 7 major lyssavirus species. The entire detection procedure takes about 8 h, including 1 h for RNA extraction, 3 h for RT-nPCR labeling, h for hybridization, and 30 min for washing and scanning.

Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC staining is commonly used in many research and clinical applications.

This Practical Guidance for Clinical Microbiology document on the laboratory diagnosis of parasites from the gastrointestinal tract provides practical information for the recovery and identification of relevant human parasites. The document is based on a comprehensive literature review and expert consensus on relevant diagnostic methods.

Cerebrospinal fluid (CSF) is a clear, watery liquid that flows around the brain and spinal cord, surrounding and protecting them.

CSF testing is performed to evaluate the level or concentration of different substances and cells in CSF in order to diagnose conditions affecting the brain and spinal cord (central nervous system).CSF is produced and secreted by the choroid plexus, a special tissue.

FATF: Diagnosing fat malabsorption due to pancreatic or intestinal disorders Monitoring effectiveness of enzyme supplementation in certain malabsorption disorders This test is not useful for differentiating among pancreatic diseases.

First, the sexual assault nurse should conduct nucleic acid amplified testing for chlamydia and gonorrhea. Next, the nurse should check for trichomoniasis via a wet mount and culture or point-of-care testing of a vaginal swab specimen. After this, a serum sample for HIV. (c) Photograph of the gross specimen reveals a homogeneous, yellow, soft mass with a distinct capsular margin.

(d) Photomicrograph (original magnification, × ; H-E stain) shows adipocytes with small nuclei that vary in cell size and shape and histiocytes (arrowheads) that engulf the necrotic fat cells. These features should not be confused.Specimens from critically ill patients can be given priority over other specimens if this would lead to better clinical management.

If a specimen requires a rapid diagnosis, a means to reach the appropriate clinician (e.g., a beeper number) must be included. A rush diagnosis for one case results in a delay for non-rush cases. Therefore. Adipose tissue-derived mesenchymal stem cells (ASCs) offer a promising cell source for therapeutic applications in musculoskeletal disorders.

The appropriate selection of ASCs from various fat depots for cell-based therapy is challenging. The present study aims to compare stemness and multipotency of ASCs derived from retroperitoneal (RP), subcutaneous (SC), and lipoma (LP) fat to .